| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Methanosphaera cuniculi | GGAGATGATGGACTAGGACCA | TTCCCCAGGTGTTGTGTCAAA | 58.04 | 59.99 | 198 | 135 |
100.00%
|
100.00%
|
| Primer 12 | Methanosphaera cuniculi | GCAGTAGCATTCATGGATCCA | TTAGCCCATGGTCCACGAG | 58.15 | 59.10 | 164 | 135 |
100.00%
|
100.00%
|
| Primer 13 | Methanosphaera cuniculi | GCAGTAGCATTCATGGATCCA | GTTAGCCCATGGTCCACGAG | 58.15 | 60.46 | 165 | 135 |
100.00%
|
100.00%
|
| Primer 14 | Methanosphaera cuniculi | GGAGATGATGGACTAGGACCA | TTCCCCAGGTGTTGTGTCA | 58.04 | 58.69 | 198 | 132 |
100.00%
|
100.00%
|
| Primer 15 | Methanosphaera cuniculi | GGAGATGATGGACTAGGACCA | TTTCCCCAGGTGTTGTGTCA | 58.04 | 59.37 | 199 | 132 |
100.00%
|
100.00%
|
| Primer 16 | Methanosphaera cuniculi | GGAGATGATGGACTAGGACCA | ATTTCCCCAGGTGTTGTGTCA | 58.04 | 59.78 | 200 | 132 |
100.00%
|
100.00%
|
| Primer 17 | Methanosphaera cuniculi | GCAGTAGCATTCATGGATCCA | TGTTAGCCCATGGTCCACG | 58.15 | 59.70 | 166 | 132 |
100.00%
|
100.00%
|
| Primer 18 | Methanosphaera cuniculi | GCAGTAGCATTCATGGATCCA | ATGTTAGCCCATGGTCCACG | 58.15 | 60.11 | 167 | 132 |
100.00%
|
100.00%
|
| Primer 19 | Methanosphaera cuniculi | TGTCTTCCACGTGTTGGTGT | ACACCAGCTTGAACCGGAT | 59.75 | 59.24 | 176 | 130 |
100.00%
|
100.00%
|
| Primer 20 | Methanosphaera cuniculi | GGACTTTCAAGCGTTGCGG | GGAGTATCACCTGTTGTGTCC | 60.08 | 58.03 | 251 | 130 |
100.00%
|
100.00%
|